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Cerebrospinal fluid samples of patients with Parkinson’s disease and healthy controls were used in this study. After tryptic digestion, all samples were spiked with indexed retention time (iRT) peptides and were measured using a DIA mass spectrometry approach. The CSF samples were prepared for digestion using consecutively RapiGestTM SF Surfactant, DTT and iodoacetamide. Then, proteolytic digestion was performedby trypsin solution and stopped by TFA. Dried peptides were resuspended in TFA. The ...

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