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The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest by proximity-dependent biotinylation. Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry that is suitable to specifically map the content of autophagosomes and potentially other transient vesicles without the need of subcellular fractionation. By combining live-cell biotinylation with proteinase K digestion of cell homogenates, proteins enriched ...

We compared transcriptomes of qNSCs and aNSCs. Quiscent neural stem cells were isolated 3 days after intraventricular injection of either aCSF or miR204 specific antagomers using described protocol (Fischer et al., 2011) and their transcriptome was analyzed

Examination of cell composition and astrocyte to neuron reprogramming in murine striatal tissue.