Data files

The crosstalk between brain infiltrating T cells and microglia in response to stroke remains elusive. Benakis et al. report that transcriptional signature of the stroke-associated microglia is reprogrammed by distinct T cell subpopulations. Engineered T cells overexpressing IL-10 administered four hours after stroke reinitiate microglial transcriptomic profile inducing a pro-regenerative environment. CD45+CD11b+ cells sorted from the ipsilateral hemisphere in naïve mice or 5 d after stroke in WT ...

Comparison of TNFα activated BMDM from B6.129P2-Apoetm1Unc/J mice treated with the inhibitors TMP195 or TPCA-1. Cells were treated for 1h with the respective inhibitors, followed by 24h stimulation with TNFα and subsequently lysed in Trizol.

pAstros were transduced with control or Ngn2-expressing retrovirus. 2DPT, media was replaced with differentiation media in absence of presence of AMG; 20DPT, DsRed-positive cells were sorted, barcoded with CMOs and analyzed by scRNAseq via 10XGenomics platform.

pAstros were transduced with control or Ngn2-expressing retrovirus. 2DPT, media was replaced with differentiation media in absence of presence of AMG; 5DPT, DsRed-positive cells were sorted, barcoded with CMOs and analyzed by scRNAseq via 10XGenomics platform

Human IPSCs from control or patients with NDUFS4 mutations were differentiated into proliferating and non-proliferating astrocytes. Three control and 3 patients lines were analyzed. Per each line, 3 biological replicates were collected at 3 stages: IPSC, proliferating astrocyte and non-proliferating astrocytes.

To determine whether CD8+ T cells contribute to oligodendrocyte and myelin pathology in 5xFAD mice, we treated 6-months old 5xFAD mice with antibodies against CD8. For the depletion of CD8+ T cells mice, 5xFAD mice aged 6 months were intraperitoneally injected with anti-CD8 antibody (BioXCell, BP0061) and their respective isotype controls (BioXCell, BE0119) twice a week for a total of 6 weeks.For 10X genomic experiments, mice were deeply anesthetized and perfused with cold PBS. Each brain was ...

Brain sections were collected from mouse model of amyloidosis and analyzed by MERFISH