Data files

scRNA-seq. Library preparation: Smart-seq2; alignement: to UCSC hg38 using HISAT2; gene counts: featureCounts (Liao et al.); single-cell analysis: Seurat // PRJNA513835 - SRP180896

Comparison of DNA accessibility by Omni-ATAC-seq in sorted effector i- and m-T cells (mD2RED CD4+ CD44high) and naive i- and m-T cells (mD2RED CD4+ CD44int) from spleen

We sorted i- and m-T cells (mD2REDCD4+CD44high) from lymph nodes and spleens of 2D2 x TH PhamT mice and performed RNAseq

Here, we use in vivo labeling at defined anatomical sites for "provance" tracking of immune cells across compartmental borders in the context of experimental autoimmune encephalomyelitis (EAE). Specifically, we labeled T cells in mesenteric or inguinal lymph nodes (LNs) of T cell conditional mitoDendra2 reporter mice via photoconversion at disease onset and re-isolated photoconverted T cells form the LNs, spleen and CNS two days later and we performed scRNA-seq on them. Our experimental system ...

Single-cell transcriptome profiling of disease-related lymphocytes in patients with multiple sclerosis

The human lysosomal polypeptide ABC transporter TAPL (ABC subfamily B member 9, ABCB9) transports 6–59 amino-acids-long polypeptides from the cytosol into lysosomes. The subcellular localization of TAPL depends solely on its N-terminal transmembrane domain TMD0, which lacks conventional targeting sequences. However, the intracellular route and the molecular mechanisms that control TAPL localization remain unclear. Here, we delineated the route of TAPL to lysosomes and investigated the determinants ...