Region-specific astrocyte heterogeneity in the white matter reveals region-specific astrogenesis Version 1
External LinkAstrocyte heterogeneity has been extensively studied, but our understanding of white matter (WM) astrocytes and their distinctions from grey matter (GM) astrocytes remains limited. We directly compared astrocytes from cortical GM, WM, and the subependymal zone using scRNA-seq and spatial transcriptomics of murine forebrain sections. The comparison revealed significant differences between WM and GM astrocytes, including cytoskeletal and metabolic hallmarks specific to WM astrocytes. Furthermore, we identified two clusters of WM astrocytes, one with shared gene expression across regions and species, observed in our scRNA-seq of cerebellar WM astrocytes and human cortical WM astrocytes, highlighting a common signature across regions and species. The other cluster of WM astrocytes was region-specific and characterized by elevated expression of progenitor/proliferation genes. Functional experiments confirmed astrocyte proliferation in telencephalic WM, but not in cerebellar WM, suggesting that WM may be a novel source of adult-generated astrocytes, potentially contributing to the GM population.
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Created: 27th Nov 2025 at 10:18
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Version 1 (earliest) Created 27th Nov 2025 at 10:18 by Aditi Methi
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Projects: SyNergy: Published Datasets, SyNergy: Unpublished Datasets
Institutions: LMU Klinikum
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Neurological diseases are on the rise – and as societies age, they affect an ever-increasing number of people, not only in Europe, but worldwide.
The Munich Cluster for Systems Neurology (SyNergy) investigates how complex neurological diseases such as Alzheimer's disease, stroke, and multiple sclerosis develop. Even though these diseases differ in their clinical manifestations, overlapping mechanisms are involved in their development. For example, the immune system gets activated in dementia, ...
Projects: SyNergy: Published Datasets, SyNergy: Unpublished Datasets
Web page: https://www.synergy-munich.de
This project serves as a centralized repository for omics datasets published by research groups within the SyNergy Cluster. It encompasses investigations such as proteomics and transcriptomics, which are further divided into individual studies led by SyNergy members. Each study is linked to relevant publications, assays and data files (with links to external repositories).
To explore investigations and their associated studies in more detail, please visit the 'Related items' tab on the Project ...
Programme: Munich Cluster for Systems Neurology (SyNergy)
Public web page: Not specified
Submitter: Rainer Malik
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Astrocyte heterogeneity has been well explored, but our understanding of white matter (WM) astrocytes and their distinctions from gray matter (GM) astrocytes remains limited. Here, we compared astrocytes from cortical GM and WM/corpus callosum (WM/CC) using single-cell RNA sequencing and spatial transcriptomics of the murine forebrain. The comparison revealed similarities but also significant differences between WM and GM astrocytes, including cytoskeletal and metabolic hallmarks specific to WM ...
Submitter: Aditi Methi
Investigation: Transcriptomics (Published)
Assays: Expression profiling: Spatial Transcriptomics (mouse), Expression profiling: scRNA-seq (mouse)
Submitter: Aditi Methi
Assay type: Transcriptomics
Technology type: Sequencing
Investigation: Transcriptomics (Published)
