Identification and validation of a tear fluid-derived protein biomarker signature in patients with Amyotrophic Lateral Sclerosis Version 1
External LinkThe diagnosis of Amyotrophic Lateral Sclerosis (ALS) primarily relies on clinical findings and remains challenging, particularly in the early stages of the disease, where characteristic symptoms may be subtle and nonspecific. Therefore, the development of disease-specific and clinically validated biomarkers is crucial to enhance diagnostic precision and optimize patient management. Here, we explored tear fluid (TF) as a promising biomarker source for ALS, given its accessibility, prior evidence of discriminative power in other neurodegenerative diseases, and its anatomical proximity to the brainstem as an important site of neurodegeneration. Using a discovery-based approach, we profiled protein abundance in TF of 49 ALS patients and 54 controls via data-independent acquisition mass spectrometry (DIA-MS) Biostatistical analysis and machine learning identified a signature of six proteins with diagnostic potential (AUROC = 66.2%).
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Created: 26th Nov 2025 at 13:42
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Version 1 (earliest) Created 26th Nov 2025 at 13:42 by Aditi Methi
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Projects: SyNergy: Published Datasets, SyNergy: Unpublished Datasets
Institutions: LMU Klinikum
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Neurological diseases are on the rise – and as societies age, they affect an ever-increasing number of people, not only in Europe, but worldwide.
The Munich Cluster for Systems Neurology (SyNergy) investigates how complex neurological diseases such as Alzheimer's disease, stroke, and multiple sclerosis develop. Even though these diseases differ in their clinical manifestations, overlapping mechanisms are involved in their development. For example, the immune system gets activated in dementia, ...
Projects: SyNergy: Published Datasets, SyNergy: Unpublished Datasets
Web page: https://www.synergy-munich.de
This project serves as a centralized repository for omics datasets published by research groups within the SyNergy Cluster. It encompasses investigations such as proteomics and transcriptomics, which are further divided into individual studies led by SyNergy members. Each study is linked to relevant publications, assays and data files (with links to external repositories).
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The diagnosis of Amyotrophic Lateral Sclerosis (ALS) remains challenging, particularly in early stages, where characteristic symptoms may be subtle and nonspecific. The development of disease-specific and clinically validated biomarkers is crucial to optimize diagnosis. Here, we explored tear fluid (TF) as a promising ALS biomarker source, given its accessibility, anatomical proximity to the brainstem as an important site of neurodegeneration, and proven discriminative power in other neurodegenerative ...
Submitter: Aditi Methi
Assay type: Proteomics
Technology type: Mass Spectrometry
Investigation: Proteomics (Published)
